Calendário de Defesas e Seminários

Defesa de Tese - Doutorado
Data: Segunda-feira, 19 de Agosto de 2019 (10:30 - 23:59)

The influence of a Tryptophan deficient diet in conjunction with polymorphisms of the Tryptophan Hydroxylase 2 (TPH2) gene in the development of depression.

Orientador: Dr.  José Artur Bogo Chies
Coorientador:  Dr.  Andrés Delgado Cañedo
Banca  Examinadora:  Dra. Lavínia Schüler-Faccini e Dra. Jacqueline Escobar Piccoli Anfiteatro do Departamento  de Genética, Prédio 43312, Campus do Vale/UFRGS

 Depression is thepsychopathology caused by neurotransmissionalterations, the main one includes the reduction of serotonin levels causing mood swings and anxiety. One of the main genetic factor in its study is the polymorphisms of the tryptophan hydroxylase 2 (TPH 2) enzyme,a major limiting enzyme in the serotonin synthesis pathway.

Aims:  Assessthe influence of a tryptophan deficient diet together with TPH 2polymorphisms inthe development of depression.

Methodology:We conducted a case-control study. The sampleswererandomly selected and were comprised of adult individuals aged from 20 years and classified in the Center of Psychosocial Care (CAPS) of Alegrete. Samples were collectedbetweenJanuary andDecember 2017. Individuals were invited to participate in the survey as controls after judicious selection. It was applied a 24-Hour Dietary Recallform and often fed with the tryptophan-rich foods in order to assess the amount of tryptophan ingested to the comparison of the data. The anthropometric assessment included Body Mass Index (BMI) measurements, using Beck inventory. It was collected a sample of 5 ml of peripheral blood DNA extraction using the PureLink Genomic DNA Mini Kit following the manufacturer's recommendations. Three SNPs (rs4570625, rs11179000, rs17110747) are being genotyped using the TaqMansystem. The results were considered significant when p ≤ 0.05.

Preliminary results: We evaluated 398 individuals with an average age of 46.4 ± 15.8 years (range 18 to 84 years), mostly female gender (78.6%) and young adults (77.4%). Depressive patients were diagnosed with mild (35.8%) or moderate depression (33.0%) (p< 0.001), and would ingest lower amounts of tryptophan when compared to non-depressive (p 0.001). It was observed that lower consumption of the Tryptophan was associated with a higher prevalence of depressive symptoms (p 0.001)

Perspectives: Thus, our findings suggest an association between a Tryptophan deficient diet and the depression pathology. We will genotypethree SNPs of the Tryptophan Hydroxylase 2 (TPH2) gene in order to test if the mutant variants can contribute to the development of the pathology.

Local  Anfiteatro do Departamento de Genética, Prédio 43312, Campus do Vale/UFRGS
Contato: PPGBM - Av. Bento Gonçalves, 9500 - Prédio 43312 M - Telefone: (51)3308-6722 / E-mail:
por Tamara Moch