{"id":64,"date":"2018-05-30T12:41:00","date_gmt":"2018-05-30T15:41:00","guid":{"rendered":"https:\/\/www.ufrgs.br\/lapcom\/portfolio\/"},"modified":"2026-04-29T13:06:52","modified_gmt":"2026-04-29T16:06:52","slug":"portfolio","status":"publish","type":"page","link":"https:\/\/www.ufrgs.br\/lapcom\/portfolio\/","title":{"rendered":"Protocols"},"content":{"rendered":"<p>Protocols available at:<\/p>\n<p><strong><a href=\"https:\/\/www.protocols.io\/researchers\/angelo-piato\">https:\/\/www.protocols.io\/researchers\/angelo-piato<\/a><\/strong><\/p>\n\n\n<ul class=\"wp-block-list\">\n<li>TRIzol-Based RNA Extraction in Adult Zebrafish<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.4r3l21dp4g1y\/v1\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.4r3l21dp4g1y\/v1<\/strong><\/a><\/p>\n\n\n\n<ol class=\"wp-block-list\"><\/ol>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Gelatin coating of microscope slides for zebrafish brain tissue sections<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.e6nvw4rpzlmk\/v1\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.e6nvw4rpzlmk\/v1<\/strong><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Measuring shoal cohesion from video frames in ImageJ using the Interfish Distance plugin<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.j8nlkwz7wl5r\/v1\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.j8nlkwz7wl5r\/v1<\/strong><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Quantification of nonprotein sulfhydryl groups (NPSH) optimized for zebrafish brain tissue V.2<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.bx8tprwn\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.bx8tprwn<\/strong><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Antioxidant activity by FRAP assay:<em>&nbsp;in vitro<\/em>&nbsp;protocol<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.btqrnmv6\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.btqrnmv6<\/strong><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Quantification of nonprotein sulfhydryl groups (NPSH) optimized for zebrafish brain tissue V.2<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.bx8tprwn\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.bx8tprwn<\/strong><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Quantification of&nbsp;thiobarbituric acid reactive species (TBARS) optimized for zebrafish brain tissue<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.bjp8kmrw\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.bjp8kmrw<\/strong><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Protein quantification protocol optimized for zebrafish brain tissue (Bradford method)<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.bjnfkmbn\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.bjnfkmbn<\/strong><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>How to prepare zebrafish brain tissue samples for biochemical assays<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.bjkdkks6\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.bjkdkks6<\/strong><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Antioxidant activity by Deoxyribose assay:&nbsp;<em>in vitro&nbsp;<\/em>protocol<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.btjdnki6\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.btjdnki6<\/strong><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Antioxidant activity by DPPH assay:&nbsp;<em>in vitro<\/em>&nbsp;protocol<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.btbpnimn\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.btbpnimn<\/strong><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Antioxidant activity by reduced glutathione (GSH) assay:&nbsp;<em>in vitro<\/em>&nbsp;protocol<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.btaynifw\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.btaynifw<\/strong><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Glutathione reductase (GR) activity assessment for zebrafish brain tissue<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.bsuuneww\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.bsuuneww<\/strong><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Glutathione peroxidase (GPx) activity assessment for zebrafish brain tissue<\/li>\n<\/ul>\n\n\n\n<p><a href=\"https:\/\/dx.doi.org\/10.17504\/protocols.io.bsujneun\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>dx.doi.org\/10.17504\/protocols.io.bsujneun<\/strong><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Protocols available at: https:\/\/www.protocols.io\/researchers\/angelo-piato dx.doi.org\/10.17504\/protocols.io.4r3l21dp4g1y\/v1 dx.doi.org\/10.17504\/protocols.io.e6nvw4rpzlmk\/v1 dx.doi.org\/10.17504\/protocols.io.j8nlkwz7wl5r\/v1 dx.doi.org\/10.17504\/protocols.io.bx8tprwn dx.doi.org\/10.17504\/protocols.io.btqrnmv6 dx.doi.org\/10.17504\/protocols.io.bx8tprwn dx.doi.org\/10.17504\/protocols.io.bjp8kmrw dx.doi.org\/10.17504\/protocols.io.bjnfkmbn dx.doi.org\/10.17504\/protocols.io.bjkdkks6 dx.doi.org\/10.17504\/protocols.io.btjdnki6 dx.doi.org\/10.17504\/protocols.io.btbpnimn dx.doi.org\/10.17504\/protocols.io.btaynifw dx.doi.org\/10.17504\/protocols.io.bsuuneww dx.doi.org\/10.17504\/protocols.io.bsujneun<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-64","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.ufrgs.br\/lapcom\/wp-json\/wp\/v2\/pages\/64","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ufrgs.br\/lapcom\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ufrgs.br\/lapcom\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ufrgs.br\/lapcom\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ufrgs.br\/lapcom\/wp-json\/wp\/v2\/comments?post=64"}],"version-history":[{"count":11,"href":"https:\/\/www.ufrgs.br\/lapcom\/wp-json\/wp\/v2\/pages\/64\/revisions"}],"predecessor-version":[{"id":830,"href":"https:\/\/www.ufrgs.br\/lapcom\/wp-json\/wp\/v2\/pages\/64\/revisions\/830"}],"wp:attachment":[{"href":"https:\/\/www.ufrgs.br\/lapcom\/wp-json\/wp\/v2\/media?parent=64"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}