{"id":15617,"date":"2024-09-30T15:47:11","date_gmt":"2024-09-30T18:47:11","guid":{"rendered":"https:\/\/www.ufrgs.br\/ppgbm\/?p=15617"},"modified":"2024-09-30T15:53:11","modified_gmt":"2024-09-30T18:53:11","slug":"02-10-seminario-de-dados-mestrado","status":"publish","type":"post","link":"https:\/\/www.ufrgs.br\/ppgbm\/02-10-seminario-de-dados-mestrado\/","title":{"rendered":"02\/10 &#8211; SEMIN\u00c1RIO DE DADOS (Mestrado) &#8211; GUSTAVO MAIA LE\u00c3O"},"content":{"rendered":"<p><strong>O2 de outubro de 2024 (quarta-feira)\u00a0 \u00a012h<\/strong><\/p>\n<p><strong>SEMIN\u00c1RIO DE DADOS (Mestrado)<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>GUSTAVO MAIA LE\u00c3O\u00a0<\/strong><\/p>\n<p><strong>Unraveling the Genetic and Biochemical Diversity of Anthocyanins in\u00a0<\/strong><em><strong>Eugenia uniflora<\/strong><\/em><strong>: A Comparative Study of Restinga and Riparian Forest Populations<\/strong><\/p>\n<p>Orientadora: Dra. Andreia Carina Turchetto Zollet<\/p>\n<p>Avaliador: Dr. En\u00e9as Ricardo Konzen<\/p>\n<p>O2 de outubro\u00a0de\u00a02024 (quarta-feira)<\/p>\n<p><strong>12h<\/strong><\/p>\n<p><strong>Presencial:\u00a0<\/strong>Anfiteatro do\u00a0Departamento\u00a0de\u00a0Gen\u00e9tica da UFRGS (Campus do Vale, pr\u00e9dio 43312)<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Abstract<\/strong><\/p>\n<p>Anthocyanins are plant pigments derived from the phenylpropanoid pathway, responsive to various environmental stresses, with potential implications for species adaptation to different conditions and natural selection.\u00a0<em>Eugenia uniflora<\/em>\u00a0is a widely distributed plant species in the Atlantic Forest, local adapted in different habitats such as riparian forest and restinga. Several mechanisms may contribute to the adaptation of plants to heterogeneous environmental conditions, including the diversity of anthocyanin compounds, which can provide adaptive value by enhancing the plant&#8217;s ability to cope with various stresses such as UV radiation, drought, and pathogen attack. This study aims to explore the genetic and biochemical variability of anthocyanins in natural populations of this species, investigating the possible adaptations of the species to heterogeneous environments. The central objective of this work is to analyze and characterize the biochemical composition of these anthocyanins in parental populations of\u00a0<em>E. uniflora<\/em>\u00a0from restinga and riparian forest, as well as their transplanted progenies. Additionally, the study seeks to identify,\u00a0<em>in silico<\/em>, the genes involved in the biosynthesis of these pigments, using phylogenetic analyses to understand the evolutionary relationship between the genes and to evaluate the differential expression (DEGs) of these genes under environmental stress conditions. Phylogenetic reconstructions were carried out with various species from the phylum Streptophyta. In the genome of\u00a0<em>E. uniflora<\/em>, three paralogs of the F3H gene (Flavanone 3-Hydroxylase), two of the F3\u2019H gene (Flavonoid 3\u2019-Hydroxylase), and single copies of the F3\u20195\u2019H (Flavonoid 3\u20195\u2019-Hydroxylase), DFR (Dihydroflavonol 4-Reductase), and ANS (Anthocyanidin Synthase) genes were identified, all components of the biosynthetic pathway, including EBG (Early Biosynthetic Genes) and LBG (Late Biosynthetic Genes). Bioinformatic analyses showed that almost all\u00a0<em>E. uniflora<\/em>\u00a0paralogs clustered with orthologs from\u00a0<em>Eucalyptus grandis<\/em>, confirming the phylogenetic relationship as both species belong to the Myrtaceae family. The analysis of conserved motifs and domains revealed high similarity with species functionally characterized for the selected genes, such as\u00a0<em>Vitis vinifera<\/em>\u00a0and\u00a0<em>Arabidopsis thaliana<\/em>. The progenies of\u00a0<em>E. uniflora<\/em>\u00a0were subjected to drought conditions, which influenced the differential expression of several genes, with particular emphasis on the F3H gene (g23674), which was both upregulated and downregulated under drought conditions. Future perspectives include the use of additional computational tools to better characterize the predicted genes, as well as molecular and quantitative analyses of anthocyanins via HPLC-qToF-MS and their correlation with the environments in which they occur. This information will be valuable for strengthening the conservation of genetic resources, identifying differentiated biochemical profiles among populations, and understanding the mechanisms of species adaptation.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>O2 de outubro de 2024 (quarta-feira)\u00a0 \u00a012h SEMIN\u00c1RIO DE DADOS (Mestrado) &nbsp; GUSTAVO MAIA LE\u00c3O\u00a0 Unraveling the Genetic and Biochemical Diversity of Anthocyanins in\u00a0Eugenia uniflora: A Comparative Study of Restinga [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[108],"tags":[],"class_list":["post-15617","post","type-post","status-publish","format-standard","hentry","category-seminarios-e-defesas"],"_links":{"self":[{"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/posts\/15617","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/comments?post=15617"}],"version-history":[{"count":2,"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/posts\/15617\/revisions"}],"predecessor-version":[{"id":15619,"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/posts\/15617\/revisions\/15619"}],"wp:attachment":[{"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/media?parent=15617"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/categories?post=15617"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/tags?post=15617"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}