{"id":18681,"date":"2026-10-06T11:53:53","date_gmt":"2026-10-06T14:53:53","guid":{"rendered":"https:\/\/www.ufrgs.br\/ppgbm\/?p=18681"},"modified":"2026-10-06T14:08:18","modified_gmt":"2026-10-06T17:08:18","slug":"07-10-2026-seminario-de-dados-mestrado-eduarda-ribeiro-koehler","status":"publish","type":"post","link":"https:\/\/www.ufrgs.br\/ppgbm\/07-10-2026-seminario-de-dados-mestrado-eduarda-ribeiro-koehler\/","title":{"rendered":"07\/10\/2026 – SEMIN\u00c1RIO DE DADOS (Mestrado) – EDUARDA RIBEIRO KOEHLER"},"content":{"rendered":"

SEMIN\u00c1RIO\u00a0DE\u00a0DADOS\u00a0(Mestrado)<\/strong><\/p>\n

 <\/p>\n

EDUARDA RIBEIRO KOEHLER<\/strong>\u00a0<\/strong><\/p>\n

Oxidative Stress and mariner <\/em>Transposable Element Mobilization in Drosophila simulans<\/em><\/strong><\/p>\n

Orientador: Dr. \u00c9lgion L\u00facio da Silva Loreto<\/p>\n

Avaliadora: Dra. Mar\u00edndia Depr\u00e1<\/p>\n

07 de outubro de 2026 (quarta-feira)<\/p>\n

12h<\/strong><\/p>\n

Apresenta\u00e7\u00e3o somente na modalidade remota<\/strong><\/p>\n

Sala virtual:<\/strong>\u00a0https:\/\/conferenciaweb.rnp.br\/ufrgs\/ppgbm<\/a><\/p>\n

 <\/p>\n

Abstract<\/strong><\/p>\n

Transposable elements (TEs), also known as \u201cjumping genes,\u201d are DNA sequences capable of moving to different genomic locations and constitute a substantial fraction of eukaryotic genomes. Their mobilization may contribute to genomic instability, changes in gene expression, cellular aging and disease, potentially affecting organismal fitness. Although several studies have associated TE mobilization with stressful conditions, it remains unclear whether this response represents a general consequence of cellular stress or depends on the specific type of stressor and the damage it induces. The mariner <\/em>TE provides an appropriate model for investigating this relationship because its somatic excision (SE) can be phenotypically monitored in Drosophila simulans <\/em>carrying the wpch <\/em>mutation through the formation of mosaic eye. In this study, we investigated whether oxidative stress \u2013 characterized by an imbalance between the formation of reactive species and free radicals and the defense capacity of antioxidant and repair systems, potentially causing damage to biomolecules such as DNA \u2013 modulates mariner <\/em>activity and whether different oxidative stressors induce distinct responses. Male D. simulans wpch <\/em>were exposed to four chemical agents with different mechanisms of action and oxidative and\/or genotoxic potential: hydrogen peroxide (H2O2), paraquat (PQ), cyclophosphamide (CP), and ethidium bromide (EtBr). Acute and chronic exposure protocols were used to investigate biochemical, physiological and genomic responses. Oxidative stress was assessed through total reactive species production and the activities of the antioxidant enzymes catalase, superoxide dismutase, and glutathione S-transferase. Longevity was evaluated using a survival curve as a physiological outcome, while mariner <\/em>activity was assessed through the frequency of somatic excision, observed through the mosaic phenotype in the flies’ eyes. All treatments significantly increased reactive species production and altered antioxidant enzyme activities, confirming the induction of oxidative stress. In addition, all exposed groups showed significantly reduced longevity compared with the control group, demonstrating that the treatments induced relevant physiological effects. Despite these common responses, a significant increase in mariner <\/em>somatic excision was observed exclusively in EtBr exposure. Therefore, the induction of oxidative stress alone was not sufficient to promote mariner <\/em>mobilization. These results indicate that the activity of the mariner <\/em>element is not universally influenced by oxidative stress and suggest that the mobilization of transposable elements is not universal, potentially depending on the specific characteristics of the stressor and the molecular mechanisms resulting from the consequent cellular and genomic damage.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

SEMIN\u00c1RIO\u00a0DE\u00a0DADOS\u00a0(Mestrado)   EDUARDA RIBEIRO KOEHLER\u00a0 Oxidative Stress and mariner Transposable Element Mobilization in Drosophila simulans Orientador: Dr. \u00c9lgion L\u00facio da Silva Loreto Avaliadora: Dra. Mar\u00edndia Depr\u00e1 07 de outubro de […]<\/p>\n","protected":false},"author":3,"featured_media":18682,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[108],"tags":[],"class_list":["post-18681","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-seminarios-e-defesas"],"_links":{"self":[{"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/posts\/18681","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/comments?post=18681"}],"version-history":[{"count":2,"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/posts\/18681\/revisions"}],"predecessor-version":[{"id":18684,"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/posts\/18681\/revisions\/18684"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/media\/18682"}],"wp:attachment":[{"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/media?parent=18681"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/categories?post=18681"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ufrgs.br\/ppgbm\/wp-json\/wp\/v2\/tags?post=18681"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}