Abstract
Phylogenetically closely related species tend to be more similar to each other than to more distantly related ones, a pattern called phylogenetic signal. Appropriate tests to evaluate the association between phylogenetic relatedness and trait variation among species are employed in a myriad of eco-evolutionary studies. However, most tests available to date are only suitable for datasets describing continuous traits, and are most often applicable only for single trait analysis. The Mantel test is a useful method to measure phylogenetic signal for multiple (continuous, binary and/or categorical) traits. However, the classical Mantel test does not incorporate any evolutionary model (EM) in the analysis. Here, we describe a new analytical procedure, which incorporates explicitly an evolutionary model in the standard Mantel test (EM-Mantel). We run numerical simulations to evaluate its statistical properties, under different combinations of species pool size, trait type and number. Our results showed that EM-Mantel test has appropriate type I error and acceptable power, which increases with the strength of phylogenetic signal and with species pool size but depended on trait type. EM-Mantel test is a good alternative for measuring phylogenetic signal in binary and categorical traits and for datasets with multiple traits.